《植物生理学报》 2010, 46(7): 653-658

西伯利亚蓼中PsMnSOD 基因的克隆及其抗逆性检测

付畅¹ , 刘关君^2,3, 张恒¹, 杨传平^2,3,*

1 哈尔滨师范大学生命科学与技术学院, 哈尔滨 150025 ; 东北林业大学林学院2 林木遗传育种与生物技术教育部重点实验室, 3 林木遗传育种黑龙江省重点实验室, 哈尔滨 150040

通信作者：杨传平；E-mail: yangcp@nefu.edu.cn；Tel: 0451-82190006

摘　要：

采用 RACE 技术从西伯利亚蓼中克隆了锰超氧化物歧化酶基因 PsMnSOD 的 cDNA (GenBank 登录号 FJ848572)。长 为 792 bp 的 PsMnSOD cDNA 序列含有编码 234 个氨基酸的 705 bp 的开放读码框、57 bp 的 5' 非翻译区和 30 bp 的 3' 非翻 译区。构建了 PsMnSOD 基因的酵母表达载体 pYES2-PsMnSOD 并将其转化到野生型酵母菌株中。分析 PsMnSOD 基因在 酵母中的表达对酵母 SOD 酶活性的影响及其对盐胁迫、PEG 胁迫、高温和低温胁迫下抗逆性影响的结果表明, PsMnSOD 基因在酵母中表达后酵母 SOD 酶活性提高, 酵母对盐渍、PEG、高温和低温等非生物胁迫的抗逆性也增强。

关键词：西伯利亚蓼; 锰超氧化物岐化酶; 抗逆性

收稿：2009-12-13   修定：2010-05-05

资助：东北林业大学林木遗传育种与生物技术教育部重点实验室开放课题、东北林业大学林木遗传育种黑龙江省重点实验室开放课题和黑龙江省青年科学技术专项资金项目(QC06C044)。

Cloning and Identification of Stress Resistance of Superoxide Dismutase Gene PsMnSOD from Polygonum sibiricum Laxm

FU Chang¹, LIU Guan-Jun^2,3, ZHANG Heng¹, YANG Chuan-Ping^2,3,*

¹College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China;²Key Laboratory of Ministry of Education for Forestry Tree Genetic Improvement and Biotechnology, ³Key Laboratory of Heilongjiang Province for Forestry Tree Genetic Improvement, School of Forestry, Northeast Forestry University, Harbin 150040, China

Corresponding author: YANG Chuan-Ping; E-mail: yangcp@nefu.edu.cn; Tel: 0451-82190006

Abstract:

In this research, PsMnSOD cDNA (GenBank No. FJ848572) was isolated from Polygonum sibiricum by RACE technology. The nucleotide sequence of PsMnSOD cDNA is 792 bp containing an open reading frame of 705 bp encoding 234 amino acids, with a 5' untranslated region of 57 bp and a 3' untranslated region of 30 bp. The PsMnSOD gene yeast expression vector pYES2-PsMnSOD was constructed and transformed into wild yeast strain. The effects of PsMnSOD gene expression on superoxide dismutase activity and resistance to abiotic stress including salt, PEG, high temperature and low temperature of yeast were determined. The results indicated that expression of PsMnSOD gene in yeast improved superoxide dismutase activity and resistance ability to abiotic stress including salt, PEG, high temperature and low temperature.

Key words: Polygonum sibiricum; manganese superoxide dismutase; stress resistance